Transcriptomic and Proteomic Analyses of Rice Pistils during Pollination

来源 :第十届全国植物结构与生殖生物学学术研讨会 | 被引量 : 0次 | 上传用户:tanmh
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
  Pollination is a critical step that can determine the rate of seed setting.Compared with those of the self-incompatibility (SI),the mechanisms of compatible pollination are still largely unknown.Previous studies mainly focused on the gene expressional profile of stigma or pistil rather than its dynamic changes during the pollen-pistil interaction.In this study,the deep-sequencing based transcriptomic technique,iTRAQ and two-dimensional gel electrophoresis based quantitative proteomic techniques were combined to analyze the dynamic changes of gene expressional profile in rice pistil during the pollination.After bioinformatics analysis,RNA-Seq results yielded a total of 963 genes that exhibited significant (FDR<0.001) and differential (ratio value >2 or <0.5) expression.iTRAQ and 2D-MS separately yielded 145 and 41 differentially expressed proteins.Among those,we found that fine-tuning of the biochemical and physiological cellular environment is crucial for reproductive success.Our study provides a temporal and spatial gene expressional profile of pollen-pistil interactions,which will help us to obtain a more comprehensive view into the gene expression networks and biochemical pathways during the plant sexual reproductive process.
  以燕山板栗品种‘燕红’为材料,采用实时荧光定量PCR 和双波长紫外分光光度计法,对淀粉合成关键酶ADP-葡萄糖焦磷酸化酶(AGPase)、颗粒紧密结合的淀粉合成酶(GBSS)、可溶
  二倍体草莓(Fragaria vesca)为多年生草本果树。以三种二倍体草莓(“Hawaii 4”,“Reugen”,“alba”)为材料,利用高效液相色谱(HPLC-DAD)和液质联用技术(HPLC-ESI-MSn)
  The fertilization process and cytological mechanism of preventing polyspermy in the fern Osmunda japonica Thunb.are studied based on the observations of tra
  山茶属系统演化存在较大的分歧,主要存在于Sealy 的分类系统、张宏达的分类系统以及闵天禄的分类系统之间.以叶片解剖特征为属性已经成功运用到山茶属分类中,然而,叶片特